(AFB CULTURE BLOOD)
| Name
| Method
| Reference Range
| Units
|
| Specimen Source |
|
|
|
| Acid-fast Bacilli Stain |
STAIN |
No AFB Seen |
|
| Preliminary Culture |
CULTURE |
By Report |
|
| Preliminary Culture |
CULTURE |
By Report |
|
| No
| Type
| Volume
| Temperature
| Container Type
| Instructions
|
| 1 |
Sterile Container/Tube |
2 (1) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 2 |
Nasopharyngeal Swab |
1 (1) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 3 |
Stool |
2 (1) g |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Stool |
|
| 4 |
Tissue |
1 (0.5) g |
Ambient - 72 Hour(s)/Refrigerated - 7 Day(s) |
Sterile |
Place tissue & needle biopsies in Middlebrook broth/// |
| 5 |
Urine |
10 (5) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 6 |
Whole Blood ACD |
10 (5) mL |
Ambient - 24 Hour(s) |
Yellow |
|
| 7 |
Blood Culture Bottle |
|
Ambient - 24 Hour(s) |
Other |
|
| 8 |
Whole Blood Heparin |
10 (5) mL |
Ambient - 24 Hour(s) |
Green |
|
| 9 |
Whole Blood Isolator Tube |
10 (5) mL |
Ambient - 24 Hour(s) |
Black/Yell |
|
| 10 |
Bone Marrow ACD |
|
Ambient - 24 Hour(s) |
Yellow |
|
| 11 |
Bone Marrow Heparinized |
|
Ambient - 24 Hour(s) |
Green |
|
| 12 |
CSF |
3 (1.5) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 13 |
Culturette/Swab |
1 (1) mL |
Ambient - 24 Hour(s)/Refrigerated - 7 Day(s) |
Culturette |
|
| 14 |
Fluid Ascitic |
10 (5) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 15 |
Fluid |
10 (5) mL |
Ambient - 24 Hour(s)/Refrigerated - 7 Day(s) |
Sterile |
|
| 16 |
Fluid Pericardial |
10 (5) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 17 |
Fluid Peritoneal |
10 (5) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 18 |
Fluid Pleural |
10 (5) mL |
Ambient - 24 Hour(s)/Refrigerated - 7 Day(s) |
Sterile |
|
| 19 |
Fluid Synovial |
10 (5) mL |
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| 20 |
Gastric Aspirate |
|
Refrigerated - 7 Day(s)/Ambient - 24 Hour(s) |
Sterile |
|
| Setup Schedule |
Sunday-Saturday |
| Reported |
53 days |
| CPT Codes
|
87015, 87116, 87206 |
| Notes |
CPT Codes: 87116; 87206; 87015 1. Source of specimen is required, please include on requisition. 2. Specimens that are heavily contaminated with the normal flora may result in test delay. 3. AFB stain will be performed on whole blood; however, mycobacteria are often present in either very low concentrations or intermittently in peripheral blood. AFB blood stains may therefore yield false negative results. Interpret these results with caution. 4. Notify the laboratory if suspecting Mycobacterium genavense as this organism will not grow on media routinely used for Mycobacterium isolation. 5. The Mycobacterium Tuberculosis Direct (MTD), a target-amplified acid probe is available for respiratory specimens. Call technical services for details. 6. If culture is positive for Mycobacterium species, it will reflex to #2935 AFB Identification by DNA (GenProbe) or #5648 AFB Identification by (HPLC) at an additional charge. 7. If M. tuberculosis complex isolated, susceptibility testing will be performed and a fee will be charged. For other Mycobacterium species, susceptibility testing will be performed upon request. Prices might not include a handling fee. |
| Clinical
Utilities |
The laboratory diagnosis of mycobacterial diseases depends on the detection and recovery of AFB from clinical specimens. This assay employs the use of solid media and broth based media (MGIT) for rapid isolation of Mycobacterium species. Identifications are performed by commercial probes, HPLC, and/or conventional methods. Susceptibilities for M. tuberculosis and M. avium-intracellulare complex (MAC) are performed by the radiometric method and all others by conventional methods.
|
1. Collect specimen in a sterile, leakproof container.
2. For respiratory specimens, collect three sputum samples from
deep productive coughs in consecutive 8 to 24 hour intervals per CDC
guidelines (at least one should be an early morning specimen).
3. Place tissue and needle biopsies in Middlebrook broth (available
from Specialty) or in sterile saline.
4. Culturette and nasopharyngeal swab collections may result in a
potential decline in sensitivity of Mycobacterium isolation in
culture. The yield of material is limited and, due to the
hydrophobicity of Mycobacteria, the organisms may be entrapped
within the fiber matrix and not readily transition into solution or
onto media. False negative cultures are possible, especially if few
pathogens are present.
5. If gastric aspirate cannot be shipped immediately, add 100mg of
sodium bicarbonate as a buffer to neutralize any acid detrimental
to Mycobacteria.
6. Refrigerate all samples within 1 hour of collection time except for
whole blood samples.
7. Ship specimens on cold pack within 24 hours of collection. Whole
blood is shipped at ambient temperature.
|
